r - 为多个组生成具有不同颜色的ggplot2 boxplot

Question

我对 R 和 ggplot 还很陌生。

我正在尝试生成一个按两个变量排序的箱线图。就我而言，物种和实验。
到目前为止我得到了什么

ggplot(DF, aes(Species, Protein, fill=Experiment, dodge=Experiment)) +
    stat_boxplot(geom ='errorbar')+
    geom_boxplot()

是我的物种的箱线图，每个物种有 2 个条形图，每个实验一个。

我现在的问题是，是否有可能以这种方式改变颜色，每个物种都有不同的颜色，比如说，实验中这些颜色的不同阴影？

比方说，第一个物种会有一个深蓝色和浅蓝色条，第二个是深绿色和浅绿色条，等等。

数据采用 csv 文件的形式，如下所示:

Experiment,Species,Protein
Spring,D_strigosa,5.107767364
Spring,D_strigosa,8.288980741
Spring,D_strigosa,7.537376567
Spring,D_strigosa,4.811744241
Spring,D_strigosa,9.559043454
Spring,M_cavernosa,13.66759289
Spring,M_cavernosa,23.54880195
Spring,M_cavernosa,11.00790037
Spring,M_cavernosa,13.70597973
Spring,M_cavernosa,12.26970906
Spring,M_faveolata,9.559043454
Spring,M_faveolata,4.135860474
Spring,M_faveolata,4.557306615
Spring,M_faveolata,7.621872315
Spring,M_faveolata,6.016859956
Spring,M_annularis,6.294920733
Spring,M_annularis,5.163371831
Spring,M_annularis,2.177243143
Spring,M_annularis,3.55966551
Autumn,D_strigosa,3.709978392
Autumn,D_strigosa,7.192777836
Autumn,D_strigosa,5.31544441
Autumn,D_strigosa,6.578602058
Autumn,D_strigosa,5.610085559
Autumn,M_cavernosa,11.64182554
Autumn,M_cavernosa,10.44968102
Autumn,M_cavernosa,9.377389318
Autumn,M_cavernosa,10.99346107
Autumn,M_cavernosa,11.5676896
Autumn,M_faveolata,4.638054165
Autumn,M_faveolata,7.191664953
Autumn,M_faveolata,6.981827102
Autumn,M_faveolata,6.386452477
Autumn,M_annularis,4.709770404
Autumn,M_annularis,3.554071459
Autumn,M_annularis,4.596686351
Autumn,M_annularis,3.530585628

Answer 1

您应该使用 interaction()为 fill=并同时提供 Species和 Experiment内interaction() .然后用 scale_fill_manual()您可以设置 values=对于您需要的颜色(颜色数量对应于箱线图的数量)。颜色顺序首先是 Species 的颜色在 Autumn然后颜色为 Species在 Spring .

ggplot(DF, aes(Species, Protein, fill=interaction(Species,Experiment), dodge=Experiment)) +
  stat_boxplot(geom ='errorbar')+
  geom_boxplot()+
  scale_fill_manual(values=c("blue","green","red","cyan",
             + "darkblue","darkgreen","darkred","darkcyan"))