python - 使用python，opencv和libtiff无法保存tiff图像16位。生物科学

Question

我尝试使用python将tif图像保存16位，遵循以下代码，我不使用open cv做到这一点，我下载了libtiff和pylibtff库来做到这一点，但是我遇到了同样的问题，现在说:

AttributeError: 'numpy.ndarray' object has no attribute 'write_image'

有人知道发生了什么吗？同时打开cv和libtiff。

@author: Albert Parra
    """
    import numpy as np
    import cv2
    from matplotlib import pyplot as plt
    #from tifffile import imsave
    from libtiff import TIFF


img = cv2.imread('HSPl3_E7_LP6p5_Ch1-32_Z9_Maximum intensity.tif',-1)
img3= cv2.cvtColor(img,cv2.COLOR_GRAY2RGB)

img3 = (img3/256).astype('uint8')
#img = np.array(img, dtype=np.uint8) # This line only change the type, not values
#img = img/256 # Now we get the good values in 16 bit format
mask = np.zeros(img.shape[:2],np.uint8)

bgdModel = np.zeros((1,65),np.float64)
fgdModel = np.zeros((1,65),np.float64)

rect = (160,180,700,700)
cv2.grabCut(img3,mask,rect,bgdModel,fgdModel,5,cv2.GC_INIT_WITH_RECT)



mask2 = np.where((mask==2)|(mask==0),0,1).astype('uint8')
img2 = img*mask2[:,:,np.newaxis]

masked_img = cv2.bitwise_and(img,img,mask = mask)

plt.imshow(img),plt.colorbar(),plt.show()



plt.show()



img.write_image('HSPl3_E7_LP6p5_Ch1-32_Z9_withmask.tif')
#imsave('HSPl3_E7_LP6p5_Ch1-32_Z9_withmask.tif',img2)

#cv2.waitKey()

Answer 1

该错误表明您不能使用img.write_image方法，因为img是numpy矩阵。问题在以下语句中:

img.write_image('HSPl3_E7_LP6p5_Ch1-32_Z9_withmask.tif')

将以下语句替换为以下语句:

cv2.imwrite('HSPl3_E7_LP6p5_Ch1-32_Z9_withmask.tif', img)

您应该使用 cv2.imwrite使用 opencv保存图像